These data provide “proof of principle” that KNA + cells restore perfusion and correct vascular dysfunction in db/db mice. Proteomic array studies performed on retinas of db/db mice injected with either control or diabetic donor–derived KNA + cells showed correction of aberrant signaling in db/db retinas toward normal healthy retina. Transcriptomic analysis showed that differentiation of hiPSCs derived from diabetics into KNA + cells was sufficient to change baseline differences in gene expression caused by the diabetic status and reprogram diabetic cells to a pattern similar to KNA + cells derived from nondiabetic hiPSCs. KNA + cells differentiated into perfused blood vessels when implanted subcutaneously into the flank of nonobese diabetic/severe combined immunodeficient mice and when injected into the vitreous of type 2 diabetic mice ( db/db mice). KNA + cells had high clonal proliferative potential and specification into endothelial colony-forming cell (ECFCs) phenotype. ![]() Human induced pluripotent stem cells (hiPSCs) were differentiated into a specific mesoderm subset characterized by KDR +CD56 +APLNR + (KNA +) expression. Grant +21 authors +19 authors +14 authors fewer Authors Info & Affiliations Sielski, Yang Lin, Xinxin Huang, Mariana D. Fortmann, Ping Hu, Kimihiko Banno, Mohamed Jamal, , Chao Huang, Micheli S. Vieira, Nutan Prasain, , Sergio Li Calzi, Seth D. Chang-Hyun Gil, Dibyendu Chakraborty, , Cristiano P.
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